Publications – University of Copenhagen

Plantpower > Publications


Here the publications produced by this project will be added continuously.

Publications produced within this project:

[40] Callari R, Fischer D, Heider H, Weber N (2018). Biosynthesis of angelyl-CoA in Saccharomyces cerevisiae. Microbial Cell Factories, 17:72

[39] Mellor SB, Behrendorff JBYH, Nielsen AZ, Jensen PE, Pribil M (2018). Non-photosynthetic plastids as hosts for metabolic engineering. Essays in Biochemistry, EBC20170047

[38] Heskes, A. M., Sundram, T. C.M., Boughton, B. A., Bjerg Jensen, N., Hansen, N. L., Crocoll, C., Cozzi, F., Rasmussen, S., Hamberger, B., Hamberger, B., Staerk, D., Møller, B. L. and Pateraki, I. (2018), Biosynthesis of bioactive diterpenoids in the medicinal plant Vitex agnus-castus. Plant J. Accepted Author Manuscript. doi:10.1111/tpj.13822

[37] Vavitsas K, Gnanasekaran T, Østergaard Rue E, Stefánsdóttir LK, Blennow A, Crocoll C, Gudmundsson S, Jensen PE (2017). Responses of Synechocystis sp. PCC 6803 to heterologous biosynthetic pathways. Microb. Cell Fact 16:140 DOI 10.1186/s12934-017-0757-y

[36] Henriques de Jesus MPR, Nielsen AZ, Mellor SB, Matthes A, Burow M, Robinson C, Jensen PE (2017). Tat proteins as novel thylakoid membrane anchors organize a biosynthetic pathway in chloroplasts and increase product yield 5-fold. Metabolic Engineering, 44: 108-116

[35] Mellor SB, Vavitsas K, Nielsen AZ, Jensen PE (2017) Photosynthetic fuel for heterologous enzymes: the role of electron carrier proteins. Photosynthesis Research. doi: 10.1007/s11120-017-0364-0.

[34] Møller BL (2017) Solar-Driven Synthesis of Bioactive Natural Products.Chemical Engineering Progress 113 (4): 46-54.

[33]  Irini Pateraki, Johan Andersen-Ranberg, Niels Bjerg Jensen, Sileshi Gizachew Wubshet, Allison Maree Heskes, Victor Forman, Björn Hallström, Britta Hamberger, Mohammed Saddik Motawia, Carl Erik Olsen, Dan Staerk, Jørgen Hansen, Birger Lindberg Møller,and Bjoern Hamberger (2017). Total biosynthesis of the cyclic AMP booster forskolin from Coleus forskohlii. eLife 2017;10.7554/eLife.23001

[32] Nikolaj L. Hansen, Jakob N. Nissen, Björn Hamberger (2017). Two residues determine the product profile of the class II diterpene synthases TPS14 and TPS21 of Tripterygium wilfordii, Phytochemistry,

[31] Nikolaj L. Hansen, Allison M. Heskes, Britta Hamberger, Carl E. Olsen, Björn M. Hallström, Johan Andersen-Ranberg, and Björn Hamberger (2017). The terpene synthase gene family in Tripterygium wilfordii harbors a labdane-type diterpene synthase among the monoterpene synthase TPS-b subfamily The Plant Journal Volume 89, Issue 3, February 2017, Pages: 429–441, Version of Record online : 14 FEB 2017, DOI: 10.1111/tpj.13410

[30] Wubshet SG, Tahtah Y, Heskes AH, Kongstad KT, Pateraki I, Hamberger B, Møller BL, and Stærk D. (2016). Identification of PTP1B and α-glucosidase inhibitory serrulatanes from Eremophila spp. by combined use of dual high-resolution PTP1B and α-glucosidase inhibition profiling and HPLC-HRMS-SPE-NMR. Journal of Natural Products, 79, 1063-1072.
[29] Tahtah Y, Wubshet SG, Kongstad KT, Heskes AH, Pateraki I, Møller BL, Jäger AK,and Stærk D. (2016). High-resolution PTP1B inhibition profiling combined with high-performance liquid chromatography–high-resolution mass spectrometry–solid-phase extraction–nuclear magnetic resonance spectroscopy: Proof-of-concept and antidiabetic constituents in crude extract of Eremophila lucida. Fitoterapia, 110, 52-58.

[28] Luo D, Callari R, Hamberger B, Wubshet SG, Nielsen MT, Andersen-Ranberg J, Hallström BM, Cozzi F, Heider H, Lindberg Møller B, Staerk D, and Hamberger B (2016). Oxidation and cyclization of casbene in the biosynthesis of Euphorbia factors from mature seeds of Euphorbia lathyris L. Proc Natl Acad Sci U S A. 2016 Aug 23;113(34):E5082-9. doi: 10.1073/pnas.1607504113.

[27] Rasmussen RE, Erstad SM, Ramos-Martinez EM, Fimognari L, De Porcellinis AJ, Sakuragi Y. (2016) An easy and efficient permeabilization protocol for in vivo enzyme activity assays in cyanobacteria. Microb Cell Fact.  15:186.

[26] Alice J. de Porcellinis, Stephan Klähn, Lisa Rosgaard, Rebekka Kirsch, Kirstin Gutekunst, Jens Georg, Wolfgang R. Hess and Yumiko Sakuragi (2016) The Non-Coding RNA Ncr0700/PmgR1 is Required for Photomixotrophic Growth and the Regulation of Glycogen Accumulation in the Cyanobacterium Synechocystis sp. PCC 6803. Plant Cell Physiol. 57(10): 2091–2103 (2016) doi:10.1093/pcp/pcw128

[25] Randi Engelberth Rasmussen, Simon Matthé Erstad, Erick Miguel Ramos‑Martinez, Lorenzo Fimognari, Alice Jara De Porcellinis and Yumiko Sakuragi  (2016) An easy and efficient permeabilization protocol for in vivo enzyme activity assays in cyanobacteria. Microb Cell Fact, 15:186, DOI 10.1186/s12934-016-0587-3. Open Access

[24] Jagalski V, Barker R, Topgaard D, Günther-Pomorski T, Hamberger B, and Cárdenas M (2016). Biophysical study of resin acid effects on phospholipid membrane structure and properties. Biochimica et Biophysica Acta 1858: 2827–2838. DOI: 10.1016/j.bbamem.2016.08.008

[23] Cannella D, Möllers KB, Frigaard NU, Jensen PE, Bjerrum MJ, Johansen KS and Felby C (2016). Light-driven degradation of plant polysaccharides by photosynthetic pigments and a metalloprotein. Nature Comm. DOI: 10.1038/ncomms11134.

[22] Mellor S, Nielsen A, Burow M, Motawia M, Jakubauskas D, Møller BL, Jensen PE (2016). Fusion of ferredoxin and cytochrome P450 enables direct light-driven biosynthesis. ACS Chemical Biology, DOI: 10.1021/acschembio.6b00190.

[21] King BC, Vavitsas K, Khairul Ikram NKB, Schrøder J, Scharff LB, Hamberger BR,  Jensen PE and Simonsen HT (2016). In vivo assembly of DNA-fragments in the moss, Physcomitrella patens. Scientific Reports, 6:25030. DOI: 10.1038/srep25030.

[20] Nielsen AZ, Mellor SB, Vavitsas K, Wlodarczyk AJ, Gnanasekaran T, Ramos H de Jesus MP, King BC, Bakowski K, Jensen PE (2016). Extending the biosynthetic repertoires of cyanobacteria and chloroplasts. Plant Journal 87: 87-102, doi: 10.1111/tpj.13173.

[19] Gnanasekaran T, Karcher D, Nielsen AZ, Martens HJ, Ruf S, Kroop X, Olsen CE, Motawie MS, Pribil M, Møller BL, Bock R and Jensen PE (2016). Transfer of the cytochrome P450 dependent dhurrin pathway from Sorghum bicolor into tobacco chloroplasts for light-driven synthesis. Journal of Experimental Botany, 67(8):2495-506, doi:10.1093/jxb/erw067.

[18] Gnanasekaran T, Vavitsas K, Andersen-Ranberg J, Zygadlo Nielsen A, Olsen CE, Hamberger BR, and Jensen PE (2016). Heterologous expression of the isopimaric acid pathway in plants and the effect of N-terminal modifications of CYP720B4. J Biol Eng. 9:24. doi: 10.1186/s13036-015-0022-z. eCollection 2015.

[17] J. Andersen-Ranberg, KT Kongstad, MT Nielsen, NB Jensen, I Pateraki, SS Bach, B Hamberger, P Zerbe, D Stærk, J Bohlmann, BL Møller, BR Hamberger (2016) Expanding the Landscape of Diterpene Structural Diversity through Stereochemically Controlled Combinatorial Biosynthesis. Angew. Chem. Int. Ed. 2016, 55, 2142 10.1002/anie.201510650 

[16] Wlodarczyk A., Gnanasekaran T., Zygadlo Nielsen A., Zulu N.N., Mellor S.B., Bang Thøfner J.F., Olsen C.E., Mottawie M.S., Burow M., Luckner M, Pribil M., Feussner I., Møller B.L., and Jensen P.E. (2016) Metabolic engineering of light-driven cytochrome P450 dependent pathways into Synechocystis sp. PCC 6803. Metabolic Engineering. 33: 1-11. doi: 10.1016/j.ymben.2015.10.009.

The cover shows a cross section of the Coleus forskohlii root. The forskolin production occurs in the outermost cork in only a few cell layers. Cover image credits: Irini Pateraki and Helle Juel Martens. Click the image to download a pdf in higher resolution.

[15] Pateraki I, Heskes AM, Hamberger B. (2015). Cytochromes p450 for terpene functionalisation and metabolic engineering. Adv Biochem Eng Biotechnol. 2015;148:107-39. doi: 10.1007/10_2014_301. PMID: 25636487

[14] V. Jagalski, R. Barker, M.B. Thygesen, K. Gotfryd, M. Kruger, L. Shi, S. Maric, N. Bovet, M. Moulin, M. Haertlein, T. Gunther Pomorski, C.J. Loland, M. Cardenas. (2015) Grafted Biomembranes Containing Membrane Proteins - The Case for the Leucine Transporter. Soft Matter.  DOI: 10.1039/C5SM01490E

[13] Gangl D,  Zedler J,  Rajakumar PD, Martinez EMR, Riseley A, Wlodarczyk A, Purton S, Sakuragi Y, Howe CJ, Jensen PE, and Robinson C (2015) Biotechnological exploitation of microalgae. J. Experimental Botany
66 (22): 6975-6990. doi: 10.1093/jxb/erv426.

[12] Englund E, Andersen-Ranberg J, Miao R,
Hamberger B, Lindberg P
(2015). Metabolic Engineering of Synechocystis sp. PCC 6803 for Production of the Plant Diterpenoid Manoyl Oxide. ACS Synth Biol. 2015 Jul 13. PMID: 26133196 See more

[11] Patron NJ, Orzaez D, Marillonnet S, Warzecha H, Matthewman C, Youles M, Raitskin O, Leveau A, Farré G, Rogers C, Smith A, Hibberd J, Webb AA, Locke J, Schornack S, Ajioka J, Baulcombe DC, Zipfel C, Kamoun S, Jones JD, Kuhn H, Robatzek S, Van Esse HP, Sanders D, Oldroyd G, Martin C, Field R, O'Connor S, Fox S, Wulff B, Miller B, Breakspear A, Radhakrishnan G, Delaux PM, Loqué D, Granell A, Tissier A, Shih P, Brutnell TP, Quick WP, Rischer H, Fraser PD, Aharoni A, Raines C, South PF, Ané JM, Hamberger BR, Langdale J, Stougaard J, Bouwmeester H, Udvardi M, Murray JA, Ntoukakis V, Schäfer P, Denby K, Edwards KJ, Osbourn A, Haseloff J. (2015). Standards for plant synthetic biology: a common syntax for exchange of DNA parts. New Phytol. 2015 Jul 14. doi: 10.1111/nph.13532. PMID: 26171760

[10] Zedler, J.A.Z., Gangl, D., Hamberger, B. , Purton, S., and Robinson, C. (2015). Stable Expression of a Bifunctional Diterpene Synthase in the Chloroplast of Chlamydomonas reinhardtii. J. Appl. Phycol. (in press - available online). DOI 10.1007/s10811-014-0504-2

[9] Gangl, D., Zedler, J.A.Z., Włodarczyk, A., Jensen, P-E., Purton, S., and Robinson, C. (2015). Expression and membrane-targeting of an active plant Cytochrome P450 in the Chlamydomonas reinhardtii chloroplast genome. Phytochemistry, 110:22-8. doi: 10.1016/j.phytochem.2014.12.006.

[8] Lund CH, Bromley JR, Stenbæk A,
Rasmussen RE, Scheller HV, and Sakuragi Y

(2015) A reversible Renilla luciferase protein complementation assay for rapid identification of protein-protein interactions reveals the existence of an interaction network involved in xyloglucan biosynthesis in the plant Golgi apparatus. J Exp Bot,
66(1):85-97. doi: 10.1093/jxb/eru401. Epub 2014 Oct 18.

[7] Jensen PE, and Leister D (2014) Chloroplast evolution, structure and functions. F1000Prime Reports, 6:40 (doi:10.12703/P6-40).

[6] Lassen LM, 1, Nielsen AZ, Olsen CE, Bialek W, Jensen K, Møller BL, and Jensen PE (2014) Anchoring a Plant Cytochrome P450 via PsaM to the Thylakoids in Synechococcus sp. PCC 7002: Evidence for Light-Driven Biosynthesis. PLoS ONE (open access); 9(7) e102184. doi:10.1371/journal.pone.0102184 PDF

[5] Jensen PE and Leister D (2014). Cyanobacteria as an experimental platform for modifying bacterial and plant photosynthesis. Front. Bioeng. Biotechnol. 2:7. doi: 10.3389/fbioe.2014.00007

[4] Pateraki I, Andersen-Ranberg J, Hamberger B, Heskes AM, Martens HJ, Zerbe P, Bach SS, Møller BL, Bohlmann J, Hamberger B. (2014) Manoyl Oxide (13R), the Biosynthetic Precursor of Forskolin, is Synthesized in Specialized Root Cork Cells in Coleus forskohlii. Plant Physiol.164(3):1222-36. doi: 10.1104/pp.113.228429. See more here. 
Cover of the March edition (see photo).

[3] Lassen L.M., Nielsen A.Z., Ziersen B., Gnanasekaran T., Møller B.L., and Jensen P.E. (2014) Redirecting Photosynthetic Electron Flow into Light-Driven Synthesis of Alternative Products Including High-Value Bioactive Natural Compounds. ACS Synth. Biol., 3(1): 1-12. DOI: 10.1021/sb400136f 

[2] Zerbe P*, Hamberger B*, Yuen MM, Chiang A, Sandhu HK, Madilao LL, Nguyen A, Hamberger B, Bach SS, Bohlmann J. (2013) Gene discovery of modular diterpene metabolism in nonmodel systems. Plant Physiol.; 162(2):1073-91.
doi: 10.1104/pp.113.218347
*shared first authorship

[1] Hamberger B, and Bak S. (2013) Plant P450s as versatile drivers for evolution of species-specific chemical diversity. Philos Trans R Soc Lond B Biol Sci., 2013 Jan 6;368(1612):20120426. doi: 10.1098/rstb 

Other relevant publications produced within the consortium:

[4] Nafisi M, Stranne M, Zhang L, van Kan AL, and Sakuragi Y. (2014) The endo-arabinanase BcAra1 is a novel host-specific virulence factor of the necrotic fungal phytopathogen Botrytis cinerea.  MPMI. 27(8): 781-792. doi: 10.1094/MPMI-02-14-0036-R

[3] Liang X., Grue-Sørensen G., Månsson K., Vedsø P., Soor A., Stahlhut M., Bertelsen M., Engell K.M., and Högberg T. (2013) Syntheses, biological evaluation and SAR of ingenol mebutate analogues for treatment of actinic keratosis and non-melanoma skin cancer. Bioorg. Med. Chem. Lett., 23, 5624-5629. 

[2] Liang X., Grue-Sørensen G., Petersen A.K., and Högberg T. (2012) Semisynthesis of Ingenol 3-Angelate (PEP005): Efficient Stereoconservative Angeloylation of Alcohols. SYNLETT, 23(18), 2647-2652.

[1] Nielsen A.Z., Ziersen B., Jensen K., Lassen L.M., Olsen C.E., Møller B.L., and Jensen P.E. (2013) Redirecting Photosynthetic Reducing Power toward Bioactive Natural Product Syntesis. ACS Synth. Biol., 2, 308-315. Doi: 10.1021/sb300128r